4E Therapeutics Custom Synthesis and Medicinal Chemistry. Part A MNK

The CIDD will continue to provide synthesis support to 4E Therapeutics to prepare and characterize compounds to support the program goals.In our previous work. the synthesis route below has been modified and optimized to address the SAR needs of the 4E program.All novel compounds designed. SAR strategies and specific targeted compounds will be provided to the CIDD by 4E Therapeutics.The CIDD will execute synthesis routes (representative example shown below) to prepare the targeted compounds identified by 4E Therapeutics and fully characterize and deliver analogs to 4E Therapeutics for in vitro assessment.In the representative route. variations will occur in R. R1 and R2. as well as various heterocycles and amine sidechains.The CIDD at UTSA will execute the synthesis routes and prepare the targeted analogs. preparing 10-20 mg of each analog with a targeted purity of >96%. as assessed by 1H NMR and HPLC/MS.A 5-10 mg sample of the analog will be shipped to Dr. Sahn and 4E Therapeutics (along with a delivery sheet and compound supporting information) and the remaining supply of the compound will be retained as a storage sample in a designated storage area/fridge in the CIDD.Copies of NMR spectra and HPLC/MS data for compounds prepared will be provided to 4E Therapeutics as requested.The CIDD will work closely with 4E Therapeutics to prepare a sufficient number of structural analogs to support the goals of the program.