Pathogen-host cell interactions leading to Lyme disease will be interrogated at the single cell level.

Coxiella burnetii is an obligate intracellular gram-negative bacterium that causes acute Q fever and chronic infections in humans. Since there is no licensed vaccine for prevention of human Q fever in the US. the development of safe and effective prophylactic and therapeutic strategies for controlling C. burnetii infection remains an important goal for public health. However. there is a fundamental gap in the knowledge regarding the mechanisms of host immune response to C. burnetii natural infection.

For this proposal, we will assess three compounds for antiviral activity in a cell-based Lassa virus infection assay.

We propose a multicenter, non-blinded, parallel-group, randomized clinical trial (RCT) evaluating the effect of video versus direct laryngoscope use on successful first attempt tracheal intubations. Adult patients in participating EDs and ICUs who require tracheal intubation and who meet eligibility criteria will be enrolled and randomly assigned to either a video or direct laryngoscope for the first intubation attempt. The video laryngoscope group operators will use the video screen on the first attempt.

We have previously found that geriatric marmosets have a significantly reduced microbial diversity, and we have also found in pilot testing that oral fecal transplant can be safely used to alter a young animal?s microbial community. We hypothesize that loss of microbial diversity is significantly associated with the aging process, and that oral FMT may be a potential therapeutic for healthspan maintenance. Specific Aim 1: Determine if changes in the gut microbiome with aging are associated with significant changes in measures of healthspan and lifespan.

This project is a pilot study to demonstrate that ELISPOT assays are performing adequately.

PROJECT SUMMARY
 Interrogation of basic biological phenomena in the post-genome era relies heavily on high-throughput. sensitive technologies that provide molecular profiles of cells and tissues. High-dimensionality molecular measurements (e.g.. levels of mRNAs for many/all genes) can be readily accomplished in bulk samples and down to the single-cell level. producing comprehensive (???omics) profiles for given samples that address research needs. However. such techniques necessarily ignore the tissue localization of gene products in order to produce the high-dimensionality data.

This major goal of this funding is to outfit the Pathology Unit of SNPRC with a new chemistry analyzer, a new slide stainer and coverslipper, as well as seeks to purchase a state of the art whole slide scanner, which will provide a significant upgrade to research capabilities supported by Texas Biomed.