The major objective is to perform ELISPOT and ICS analysis for the UVF vaccine associated with project ImmunogenicityofSLA-LSQ adjuvant formulations in non-human primates.

19-036 This proposal is designed to determine the immunological changes induced by SIV Infection that contributes to the increased severity of mtb in infants, and the effects of early antiretroviral therapy (ART) and Immunotherapy (IDO-inhibitor treatment) on both infections by comparing viral/bacterial burdens, innate, adaptive, and pathogen specific cellular and humoral immune responses, pulmonary pathology, latency, reservoirs, and Disease Progression. Role: Sub-PI

The major goal is to identify antigen-specific T cell responses in macaques with LTBI and SIV co-infection. Here we have infected some animals with Mtb to develop LTBI, while others naturally progress to ATB. Further, some of the animals have been co-infected with SIV (n=6) and 4 of which reactivated and 2 didn't. In the years to come we will focus on generating more LTBI, ATB, R, NR animals and also perform SIV ART treatment. Eventually antigen-specific responses to ESAT-6, CFP-10, and CW will be evaluated in the peripheral as well as lung samples. Role: MPI

The objective of this project is to perform 29-plex luminex assays for the U19 project Regulatory Immune Cell Therapy, Promotion of Tolerance and Underlying Mechanisms in NHP Renal Transplantation.

To develop a method to effectively extract and inactivate nucleic acids from Select agents.

The major goal of the contract is to provide infrastructure support for the continuation of the Strong Heart Study including oversight of its data and samples. Dr. Cole will director the Strong Heart Study Genetics center and Chair the Strong Heart Study Steering Committee.

19-017 The major goal is to infect macaques that are Mtb-sigH vaccinated with heterologous Mtb and study immune correlates of protection using T cell and B cell markers. We will also perform B cell depletion experiments and assess the impact on vaccination induced protection from heterologous protection. Role: PI

The major goal is to understand the role of the iBALT in the development of a protective granuloma using both outbred mice and nonhuman primates. Role: MPI

The use modeling techniques to understand tuberculosis granulomas.

The major goal is to use the relevant NHP model of TB and treatment with Type 1 neutralizing antibodies to specifically assess the functional role of Type 1 IFNs in disease progression from latency to PTB. Role: MPI